{"id":5596,"date":"2024-05-04T21:32:13","date_gmt":"2024-05-04T21:32:13","guid":{"rendered":"http:\/\/invimed.pl\/?page_id=5596"},"modified":"2024-07-10T12:59:05","modified_gmt":"2024-07-10T10:59:05","slug":"witryfikacja-czyli-szybkie-mrozenie-zarodkow-i-gamet","status":"publish","type":"page","link":"https:\/\/invimed.pl\/en\/witryfikacja-czyli-mrozenie-zarodkow-i-gamet","title":{"rendered":"Vitrification, or rapid freezing of embryos and gametes"},"content":{"rendered":"<p>Vitrification is a cryopreservation technique for gametes and embryos. It is the preservation of biological material at extremely low temperatures. Vitrification is also known as vitrification. It differs from freezing in liquid nitrogen in that, thanks to the use of special cryoprotectants, it does not lead to the precipitation of ice crystals that could damage the cells.<\/p>\n\n\n\n<p>In recent years, techniques have emerged in the treatment of infertility by assisted reproduction methods (ART) that have revolutionised treatment and contributed to their increased effectiveness and the birth of millions of children worldwide. In addition to ICSI (the intra-cytoplasmic insertion of a sperm into an egg), a method that is a milestone in the development of assisted reproductive techniques, is the technique of cryopreservation of gametes (sperm and ova) and embryos called vitrification.<\/p>\n\n\n\n<section class=\"cta-3\">\n\t\t<div class=\"container cta-3__container\">\n\t\t<div class=\"position-relative cta-3__wrapper\">\n\t\t                    <div class=\"acf-innerblocks-container\">\n\n<h2 class=\"wp-block-heading\" id=\"konsultacja-embriologiczna\">Embryological consultation<\/h2>\n\n\n\n<p>If additional questions come to mind after reading this article, remember that all Invimed patients qualified for insemination or IVF can benefit from the&nbsp;<a href=\"https:\/\/invimed.pl\/en\/embryologist\/\">free embryological consultation. <\/a><\/p>\n\n<\/div>\n                \t\t<\/div>\n\t<\/div>\n<\/section>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"czym-jest-krioprezerwacja\">What is cryopreservation?<\/h2>\n\n\n\n<p>Cryopreservation (cryopreservation) techniques, i.e. the effective preservation of biological material at low and extremely low temperatures, are an integral part of in vitro fertilisation (IVF) treatment, and the ongoing development and increasing effectiveness of the method, mean that the importance of cryopreservation in treatment cycles continues to increase.<\/p>\n\n\n\n<p>Cryopreservation techniques are used to protect:<\/p>\n\n\n\n<section class=\"check-list\">\n\t    <header class=\"check-list__header\">\n        <div class=\"acf-innerblocks-container\">\n\n<h2 class=\"wp-block-heading\" id=\"jak-dostarczyc-lekarzowi-wyniki-badan\">How to provide the doctor with the test results?<\/h2>\n\n\n\n<p>In the case of consultations with a gynaecologist (as part of the initial infertility consultation) and an immunologist, the teleportation takes place on the basis of a history and medical records provided by the patient.<\/p>\n\n<\/div>\n    <\/header>\n    <ul class=\"mb-0 p-0 check-list__inner\">\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <div class=\"check-list__item__rte\">\n<p>diagnostic semen testing including:<\/p>\n<ul>\n<li>computer-assisted semen analysis (CASA),<\/li>\n<li>sperm DNA fragmentation testing (SCD),<\/li>\n<li>testing the degree of sperm binding to hyaluronan (HBA),<\/li>\n<li>morphology study of motile sperm organelles (MSOME),<\/li>\n<\/ul>\n<\/div>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>isolation of oocytes from material obtained by the doctor during the puncture of ovarian follicles,<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>morphological assessment of oocyte quality and the Oosight polarised imaging system,<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>preparation of ova for IVF procedures,<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>evaluation and preparation of semen for insemination and in vitro fertilisation procedures,<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>performing in vitro fertilisation procedures, i.e. IVM, IVF, ICSI\/PICSI\/IMSI\/HBIMSI (read the article:\u00a0<a href=\"https:\/\/invimed.pl\/en\/leczenie-nieplodnosci-in-vitro-metody\/\" target=\"_blank\" rel=\"noopener noreferrer\">In vitro fertilisation methods<\/a>).<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>embryo culture to the blastocyst stage (IVC),<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>performing embryo biopsies for pre-implantation genetic testing of PGT-A and PGT-M (read article:\u00a0<a href=\"https:\/\/invimed.pl\/en\/genetic-diagnosis-in-fertility-treatment\/\" target=\"_blank\" rel=\"noopener noreferrer\">Genetic diagnosis<\/a>),<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>standard monitoring of embryo development, assessment of their development and implantation potential,<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>Time Lapse Imaging monitoring of embryo development and prediction of developmental potential,<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>preparing embryos for transfer,<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>freezing, storage and thawing of semen and ovarian tissue,<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>Vitrification and heating of oocytes and embryos (read article:\u00a0<a href=\"https:\/\/invimed.pl\/en\/witryfikacja-czyli-szybkie-mrozenie-zarodkow-i-gamet\/\" target=\"_blank\" rel=\"noopener noreferrer\">Vitrification<\/a>),<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>running a germ cell and embryo bank.<\/p>\n            <\/div>\n        <\/li>\n        <\/ul>\n<\/section>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"bezplatne-konsultacje-embriologiczne-w-invimed\">Free embryology consultations at Invimed<\/h2>\n\n\n\n<p>Invimed patients can benefit from an embryology consultation from the moment they have started preparing for assisted reproductive procedures at their chosen Invimed clinic. A consultation with an embryologist does not have to be ordered by the attending physician, but may be recommended by him or her.&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"konsultacje-embriologiczne-kierowane-sa-do-pacjentow-invimed-ktorych-interesuja\">Embryology consultations are directed to Invimed patients who are interested in:<\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Embryological procedures and their effectiveness at Invimed.<\/li>\n\n\n\n<li>The course of the embryological procedure in their case and its outcome concerning, for example, the number of embryos obtained and vitrified, the quality of oocytes, sperm and embryos.<\/li>\n\n\n\n<li>Interpretation of own test results of comprehensive semen testing, SCD, MSOME, HBA and about methods to determine oocyte and embryo quality.<\/li>\n\n\n\n<li>Assisted reproduction procedures used at Invimed and their effectiveness (insemination, standard IVF, ICSI, IMSI, PICSI, hb- IMSI, ED, sperm freezing, oocyte and embryo vitrification, preimplantation genetic testing).<\/li>\n\n\n\n<li>Topics related to embryology that patients think may apply to their treatment process at Invimed.<\/li>\n\n\n\n<li>Possible reasons for failure, lying in the field of embryology (for patients who did not achieve pregnancy through the procedure or who had a miscarriage after confirmation).<\/li>\n<\/ol>\n\n\n\n<section class=\"accordion\">\n\t\t<div class=\"accordion__wrapper\">\n\t\t<header class=\"accordion__header\">\n\t\t\t<div class=\"acf-innerblocks-container\">\n\n<h2 class=\"wp-block-heading\" id=\"czemu-sluzy-krioprezerwacja\">What is the purpose of cryopreservation?<\/h2>\n\n<\/div>\n\t\t<\/header>\n\t\t\t<div class=\"accordion__content\">\n\t\t\t\t\t<div class=\"position-relative accordion__item\">\n\t\t\t\t\t\t\t<div class=\"d-flex justify-content-between align-items-start accordion__trigger accordion__trigger-js\" tabindex=\"0\">\n\t\t\t\t\t<h4 class=\"h3 item__title\">\n\t\t\t\t\t\tFurther treatment\t\t\t\t\t<\/h4>\n\t\t\t\t\t<div class=\"overflow-hidden svg-container\">\n\t\t\t\t\t\t<svg width=\"32\" height=\"32\" viewbox=\"0 0 32 32\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n\t\t\t\t\t\t\t<circle cx=\"16\" cy=\"16\" r=\"14\" fill=\"white\"\/>\n\t\t\t\t\t\t\t<path d=\"M16 10V22\" stroke=\"currentColor\" stroke-width=\"1.44\" stroke-linecap=\"round\" stroke-linejoin=\"round\"\/>\n\t\t\t\t\t\t\t<path d=\"M10 16H22\" stroke=\"currentColor\" stroke-width=\"1.44\" stroke-linecap=\"round\" stroke-linejoin=\"round\"\/>\n\t\t\t\t\t\t<\/svg>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t<div class=\"item__rte\">\n\t\t\t\t\t\t\t\t\t<p><strong>A chance for further treatment or another child in the family.<\/strong><br \/>\nBoth cryopreserved gametes (spermatozoa and ova) and embryos can be used for further treatment if the first attempt is unsuccessful, as well as for success when patients express a desire to expand their family with another member.<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<div class=\"position-relative accordion__item\">\n\t\t\t\t\t\t\t<div class=\"d-flex justify-content-between align-items-start accordion__trigger accordion__trigger-js\" tabindex=\"0\">\n\t\t\t\t\t<h4 class=\"h3 item__title\">\n\t\t\t\t\t\tSafeguarding fertility\t\t\t\t\t<\/h4>\n\t\t\t\t\t<div class=\"overflow-hidden svg-container\">\n\t\t\t\t\t\t<svg width=\"32\" height=\"32\" viewbox=\"0 0 32 32\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n\t\t\t\t\t\t\t<circle cx=\"16\" cy=\"16\" r=\"14\" fill=\"white\"\/>\n\t\t\t\t\t\t\t<path d=\"M16 10V22\" stroke=\"currentColor\" stroke-width=\"1.44\" stroke-linecap=\"round\" stroke-linejoin=\"round\"\/>\n\t\t\t\t\t\t\t<path d=\"M10 16H22\" stroke=\"currentColor\" stroke-width=\"1.44\" stroke-linecap=\"round\" stroke-linejoin=\"round\"\/>\n\t\t\t\t\t\t<\/svg>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t<div class=\"item__rte\">\n\t\t\t\t\t\t\t\t\t<p><strong>Securing fertility in the face of disease or very low ovarian reserve (AMH)<\/strong><br \/>\nIt is common practice to preserve the gametes of patients when an existing and progressive disease, e.g. cancer, and the consequent therapy the patient has to undergo, threatens to damage the function of the gonads and reproductive cells, leading to fertility problems in the future. This treatment is called fertility preservation.<br \/>\n<a title=\"Preservation of fertility\" href=\"https:\/\/invimed.pl\/en\/treatment-of-infertility\/egg-freezing\/\" target=\"_blank\" rel=\"noopener noreferrer\">Find out more about fertility preservation &gt;&gt;<\/a><\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<div class=\"position-relative accordion__item\">\n\t\t\t\t\t\t\t<div class=\"d-flex justify-content-between align-items-start accordion__trigger accordion__trigger-js\" tabindex=\"0\">\n\t\t\t\t\t<h4 class=\"h3 item__title\">\n\t\t\t\t\t\tMaterial banking\t\t\t\t\t<\/h4>\n\t\t\t\t\t<div class=\"overflow-hidden svg-container\">\n\t\t\t\t\t\t<svg width=\"32\" height=\"32\" viewbox=\"0 0 32 32\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n\t\t\t\t\t\t\t<circle cx=\"16\" cy=\"16\" r=\"14\" fill=\"white\"\/>\n\t\t\t\t\t\t\t<path d=\"M16 10V22\" stroke=\"currentColor\" stroke-width=\"1.44\" stroke-linecap=\"round\" stroke-linejoin=\"round\"\/>\n\t\t\t\t\t\t\t<path d=\"M10 16H22\" stroke=\"currentColor\" stroke-width=\"1.44\" stroke-linecap=\"round\" stroke-linejoin=\"round\"\/>\n\t\t\t\t\t\t<\/svg>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t<div class=\"item__rte\">\n\t\t\t\t\t\t\t\t\t<p><strong>Storage of biological material in the donor bank<\/strong>.<br \/>\nCryopreservation secures material deposited by anonymous donors who donate their reproductive cells (sperm and ova) to individuals who are incapable of producing their own gametes or whose reproductive functions are impaired to the extent that the processes leading to normal embryos, pregnancies and offspring are prevented.<\/p>\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<\/div>\n<\/section>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"powolne-zamrazanie-gamet-i-zarodkow\">Slow freezing of gametes and embryos<\/h2>\n\n\n\n<p>Until recently, cryopreservation of gametes and embryos was routinely carried out using what is known as 'slow freezing', a protocol that has been well known and recognised by clinical and academic centres for years. However, as this method was fraught with several imperfections, thanks to numerous studies, scientists have managed to develop a better method, vitrification.<\/p>\n\n\n\n<p>Among the shortcomings of slow freezing are:<\/p>\n\n\n\n<section class=\"check-list\">\n\t    <header class=\"check-list__header\">\n        <div class=\"acf-innerblocks-container\">\n\n<p><\/p>\n\n<\/div>\n    <\/header>\n    <ul class=\"mb-0 p-0 check-list__inner\">\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>high probability of ice formation inside the cell, or in its immediate vicinity, which contributes to cell damage.<\/p>\n            <\/div>\n        <\/li>\n            <li class=\"d-flex check-list__item\">\n            <div class=\"svg-container\">\n                <svg width=\"16\" height=\"16\">\n                    <use xlink:href=\"#icon-check-2\" \/>\n                <\/svg>\n            <\/div>\n            <div class=\"check-list__item__rte\">\n                <p>Insufficient protection of biological functions during slow cooling of the sample. In many cases, although the cells survived the process, many of them did not undertake further development after thawing.<\/p>\n            <\/div>\n        <\/li>\n        <\/ul>\n<\/section>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"witryfikacja-jako-lepsza-forma-mrozenia-kriokonserwacji\">Vitrification as a better form of freezing (cryopreservation)<\/h2>\n\n\n\n<p>The risks of ice crystal formation and impaired biological functions described above were reduced by rapid cooling of the biomaterial to low temperatures, i.e. vitrification.<\/p>\n\n\n\n<section class=\"cta-3\">\n\t\t<div class=\"container cta-3__container\">\n\t\t<div class=\"position-relative cta-3__wrapper\">\n\t\t                    <div class=\"acf-innerblocks-container\">\n\n<h2 class=\"wp-block-heading\" id=\"co-to-jest-witryfikacja\">What is vitrification?<\/h2>\n\n\n\n<p>Vitrification involves immediately bringing the temperature of the cryoprotectant solution containing the cells or embryos down to -196\u02daC, the temperature of liquid nitrogen. During this process, also known as vitrification (<em>vitrum&nbsp;<\/em>from the Latin, means glass) in the solution surrounding the cell, as well as in the cell itself, no ice crystals are produced.<\/p>\n\n<\/div>\n                \t\t<\/div>\n\t<\/div>\n<\/section>\n\n\n\n<p>Rapid exposure of biological material, protected by suitably high concentrations of cryoprotentants, to extremely low temperatures prevents the formation of a solid, i.e. ice crystals. The solution vitrified with the biomaterial, although resembling a solid (glass) in its properties, physically remains a very dense liquid.<\/p>\n\n\n\n<p><strong>Biological materials that can be vitrified avoid intracellular damage and other detrimental effects of slow cooling on cell biology.<\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"witryfikacja-%e2%80%93-najskuteczniejsze-zabezpieczenie-zarodkow\">Vitrification - the most effective embryo protection<\/h2>\n\n\n\n<p>Performed correctly and standardised, vitrification is currently the safest and most effective form of cryopreservation.<\/p>\n\n\n\n<p>Thanks to the implementation of vitrification in clinical practice, IVF has become extremely effective and the results obtained with it would have been unthinkable a dozen years ago.<\/p>\n\n\n\n<section class=\"benefits\" id=\"section-2\">\n\t\t<div class=\"benefits__wrapper\">\n\t\t<header class=\"benefits__header\">\n\t\t\t<div class=\"acf-innerblocks-container\">\n\n<h2 class=\"wp-block-heading\" id=\"dlaczego-warto-skorzystac-z-in-vitro-w-klinikach-invimed\">Why use IVF at InviMed clinics?<\/h2>\n\n<\/div>\n\t\t<\/header>\n\t\t\t<div class=\"benefits__content\">\n\t\t\t\t\t<div class=\"benefits__item has-title\">\n\t\t\t\t\t\t\t<header class=\"d-flex align-items-center item__header\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"my-0 item__title\">Survival at 99% level<\/h3>\n\t\t\t\t\t\t\t\t<\/header>\n\t\t\t\t\t\t\t\t\t\t<div class=\"item__rte\">\n\t\t\t\t\t<p><span style=\"font-size: var(--wp--preset--font-size--small);\">In the event of failure of fresh embryo transfer, the use of vitrification-protected surplus embryos created during the IVF procedure ensures a survival rate of 99%. After thawing (warming) they undertake further normal development.<\/span><\/p>\n<div class=\"article__paragraph_ico\">\n<div class=\"article__paragraph_ico-ico article__paragraph_ico-ico--small\"><\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<div class=\"benefits__item has-title\">\n\t\t\t\t\t\t\t<header class=\"d-flex align-items-center item__header\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"my-0 item__title\">Identical performance results<\/h3>\n\t\t\t\t\t\t\t\t<\/header>\n\t\t\t\t\t\t\t\t\t\t<div class=\"item__rte\">\n\t\t\t\t\t<p>For embryos that have previously been cryoprotected by vitrification, the chances of achieving a pregnancy and giving birth to a healthy baby are identical to the clinical results obtained with 'fresh' (non-cryopreserved) embryos.<\/p>\n<p>The situation is similar with vitrified oocytes, the use of which provides results characteristic of cells that have not been cryopreserved.<\/p>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<\/div>\n<\/section>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"dlaczego-mrozenie-zarodkow-i-oocytow-to-trudny-proces\">Why is freezing embryos and oocytes a difficult process?<\/h2>\n\n\n\n<p>Both embryos and oocytes themselves (the cells that give rise to egg cells) are very specific material and their cryopreservation is challenging. Their cryopreservation is difficult due to their size, shape, the additional barrier that is the transparent envelope and their biological specificity (metabolism and developmental status).<\/p>\n\n\n\n<p>All these features mean that the successful translation of cryobiology principles operating within other fields of medicine and biology is not straightforward or obvious when applied to reproductive medicine. For this reason, the invention of vitrification is an achievement comparable to the development of a method of&nbsp;<a href=\"https:\/\/invimed.pl\/en\/icsi-intracytoplasmic-sperm-injection-intra-cytoplasmic-sperm-injection\/\" target=\"_blank\" rel=\"noreferrer noopener\">ICSI<\/a>&nbsp;(the intra-cytoplasmic insertion of the sperm into the egg).<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"dlaczego-plemniki-sie-mrozi-a-nie-witryfikuje\">Why are sperm frozen and not vitrified?<\/h2>\n\n\n\n<p>The vitrification procedure is not normally used to store sperm (semen) for use in ART procedures. The specific structure of the sperm and the conditions necessary for vitrification (rapid cooling rate, minimum volume of cryoprotectant, high concentration of cryoprotectant - much higher than for freezing, and special media for the sperm) limit the validity of its use to only certain cases. Vitrification of single spermatozoa or spermatids obtained from testicular biopsies, for example, is possible. In other cases, the spermatozoa are subjected to slow freezing in liquid nitrogen vapour. This is the safest cryopreservation method for them.<\/p>","protected":false},"excerpt":{"rendered":"<p>Witryfikacja jest technik\u0105 krioprezerwacji gamet i zarodk\u00f3w. To zabezpieczenie materia\u0142u biologicznego w ekstremalnie niskich temperaturach. Witryfikacja nazywana jest te\u017c zeszkleniem. Od zamra\u017cania w ciek\u0142ym azocie r\u00f3\u017cni si\u0119 tym, \u017ce dzi\u0119ki u\u017cyciu specjalnych krioprotektant\u00f3w, nie prowadzi do wytr\u0105cania si\u0119 kryszta\u0142k\u00f3w lodu, kt\u00f3re mog\u0142yby uszkodzi\u0107 kom\u00f3rki. W ostatnich latach w leczeniu niep\u0142odno\u015bci metodami wspomaganego rozrodu (ART) pojawi\u0142y [&hellip;]<\/p>\n","protected":false},"author":25,"featured_media":256704,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-5596","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Witryfikacja, czyli szybkie mro\u017cenie zarodk\u00f3w i gamet - Invimed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Witryfikacja, czyli szybkie mro\u017cenie zarodk\u00f3w i gamet - Invimed\" \/>\n<meta property=\"og:description\" content=\"Witryfikacja jest technik\u0105 krioprezerwacji gamet i zarodk\u00f3w. To zabezpieczenie materia\u0142u biologicznego w ekstremalnie niskich temperaturach. Witryfikacja nazywana jest te\u017c zeszkleniem. Od zamra\u017cania w ciek\u0142ym azocie r\u00f3\u017cni si\u0119 tym, \u017ce dzi\u0119ki u\u017cyciu specjalnych krioprotektant\u00f3w, nie prowadzi do wytr\u0105cania si\u0119 kryszta\u0142k\u00f3w lodu, kt\u00f3re mog\u0142yby uszkodzi\u0107 kom\u00f3rki. 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