The eBIOM study, a comprehensive analysis of the endometrial microbiome for fertility support

When trying to get pregnant does not work, there is uncertainty, questions and often no clear answers. The eBIOM test is a modern diagnostic tool that helps to understand what is happening in the uterine environment. By analysing the endometrial microbiome, a unique set of bacteria present in the endometrium, we can uncover hidden causes of infertility, implantation failures or recurrent miscarriages. This makes it possible to select a more effective, personalised treatment.

eBIOM - what is the study?

The eBIOM is an advanced analysis of the endometrial microbiome, the bacterial environment of the endometrium. This delicate and precise test makes it possible to determine whether the bacterial flora is normal and to detect the presence of unfavourable bacteria that may interfere with fertility.

Modern medicine increasingly understands how the microbial balance in the uterus has a huge impact on the effectiveness of infertility treatment. Today we know that the endometrial mucosa is not completely sterile - it can be temporarily colonised by bacteria, both beneficial and those that cause chronic inflammation.

Lactobacilli (Lactobacillus spp.) predominate in a healthy microbiome. Their presence over 80% is considered physiological and supportive of reproductive processes. An imbalance of this balance (so-called dysbiosis) or the presence of pathogens (e.g. bacteria associated with chronic endometritis - CE) can affect difficulties in getting pregnant, failures during IVF procedures or miscarriages.

The eBIOM study combines two components:

eBIOM - it is  assessing the overall composition of the endometrial microbiome, i.e. detecting whether the endometrium is dominated by favourable flora (Lactobacillus) or whether unfavourable bacteria or abnormal proportions are present.

eBIOM CE - precise identification of pathogenic bacteria, including sexually transmitted pathogens that can cause chronic endometritis.

What you can read from the test result eBIOM ?

  • Percentage of Lactobacillus spp. Their low levels may suggest the need for probiotic support.
  • Presence of other non-pathogenic bacteria. Some of these have no known effect on fertility, but an excess may indicate an imbalance.
  • Detection of pathogenic bacteria. Their presence may require treatment with antibiotics according to current medical recommendations.

Types of eBIOM tests

Depending on the diagnostic needs, three variants of endometrial microbiome testing are available:

  • eBIOM
    Basic analysis of the bacterial flora of the endometrium. Allows assessment of whether beneficial bacteria of the genus Lactobacilluswhich promote fertility and create a favourable environment for embryo implantation.
  • eBIOM CE
    Focused microbiological analysis aimed at identifying pathogens associated with chronic endometritis that may affect fertility.
  • eBIOM+
    A comprehensive test that combines both of the above tests, i.e. assessment of the overall microflora composition and identification of pathogens. This is the most comprehensive option for analysing the endometrial microbiome.

Who is the eBIOM study for?

The eBiom test is particularly recommended for female patients:

  • with difficulties in getting pregnant despite normal results of other tests,
  • after unsuccessful attempts at in vitro fertilisation (IVF),
  • with a history of recurrent miscarriages,
  • with unexplained infertility,
  • in the diagnosis of chronic endometritis, which is usually asymptomatic

Through this examination, a more accurate diagnosis can be made and therapy can be planned to suit the woman's individual health situation.

Why choose an eBIOM test?

An abnormal composition of the uterine microflora, called dysbiosis, can interfere with the processes necessary for fertilisation and embryo implantation. Sometimes this problem produces no symptoms, but nevertheless significantly affects fertility.

The eBIOM study allows:

  • detect bacterial imbalances in the endometrium,
  • identify the presence of potential pathogens,
  • select appropriate treatment - probiotic or antibiotic therapy,
  • increase the effectiveness of infertility treatment through precise diagnosis.

Advantages of the eBIOM study

By choosing the eBIOM test, you gain:

  • Comprehensiveness. A combination of analysis of the overall microbiome (eBIOM) with precise pathogen identification (eBIOM CE), which provides a complete picture of the health of the endometrium.
  • Advanced technology. The study uses:
    • NGS (next-generation sequencing) for microflora analysis,
    • Real-time PCR for pathogen detection.
  • Credibility and security. The analysis is performed in a certified laboratory that meets the highest quality standards.
  • Aptly tailored therapy. 

Benefits of the eBIOM study

Performing an eBIOM test is an investment in precise and effective infertility treatment. Here are the key benefits:

  • Comprehensive assessment of the endometrial microbiome -including both fertility-promoting and fertility-disrupting bacteria.
  • Conclusions based on clinical data - the report contains practical diagnostic and therapeutic recommendations.
  • Clear and readable result - easy to interpret for the treating physician.
  • Reliability and quality - testing performed by an experienced, accredited Medicover Genetics laboratory, using state-of-the-art methods.

How to carry out the test?

Testing the endometrial microbiome is safe, relatively quick and does not require hospitalisation.

  • The sample should be taken - in the period after the end of menstrual bleeding but before the period of the expected next menstruation (usually between days 8 and 25 of the cycle).
  • The sample is taken from the endometrium using a special sterile kit. The whole procedure is similar to taking a swab and takes only a few minutes.
  • The sample is sent to the laboratory, where it is analysed using advanced technologies (NGS and PCR).
  • Vaginal or oral antibiotics and probiotics should be avoided 7 days prior to the test - they can disrupt the microbiome.

Results are usually available within 10 to 12 days.

How do I order a test?

The eBIOM test is available at all Invimed clinics.

How do I sign up for research at Invimed?

- Use the helpline: call 500 900 888.

- Complete the online form: fill in the form on the clinic website.

- Go in person to one of the Invimed clinics.