In vitro fertilisation methods
The essence of in vitro fertilisation is the union of a sperm and an egg under laboratory conditions
The problem of infertility is more common than you think and now affects up to 15% couples of childbearing age. For many of them, the only chance for a child is in vitro fertilisation. In forty years, more than eight million children have been born through IVF, and the number of couples who have fulfilled their dream of expanding their family continues to grow. It can work for you too. Learn about in vitro fertilisation methods.
In vitro fertilisation methods
Classical IVF fertilisation
Historically the first successfully developed method of fertilisation. It involves the placement and incubation of a sperm with an egg cell. Within a few hours, the sperm penetrates the egg cell by itself and triggers all the physiological reactions in a natural way, making the method the closest to the conditions of fertilisation that exist in the body.
The method is most commonly used in cases of mechanical infertility (obstruction or absence of fallopian tubes) and in cases where semen parameters allow its use, i.e. a male factor has been excluded or the parameters are close to normal.
The number and quality of cells collected is also a condition of use.
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ICSI fertilisation
In vitro fertilisation by ICSI is a technique that involves the injection of a pre-selected sperm directly into the egg. Docytoplasmic sperm injection (injection) is most often recommended for couples who experience difficulties conceiving due to male factor infertility, immunology, or a lack of or low conception rate after standard in vitro fertilisation.
IMSI insemination
In vitro fertilisation by IMSI is a technique involving the insertion of a sperm directly into the egg cell after selection of a sperm with a normal structure under a microscope that magnifies the image by more than six thousand times ('ordinary' microscopes normally used to observe the structure and quality of sperm magnify the image by up to one thousand two hundred times).
PICSI® insemination
In the method, spermatozoa are selected on the basis of morphological criteria and a positive and specific sperm-hyaluronate binding reaction.
Normal, motile spermatozoa react with hyaluronan through specific receptors on their head. The binding of the sperm to hyaluronate suggests that the selected sperm has undergone the entire process of spermatogenesis and spermiogenesis correctly, is biologically mature, fully functional and competent to fertilise the egg.
Fertilisation by hbIMSI
The hbIMSI method involves the injection into the egg cell of a sperm that has successfully undergone morphological selection using the MSOME technique and selection on the basis of binding to hyaluronic acid (HBA). The two-stage selection of sperm for in vitro IMSI increases the likelihood of obtaining a promising, properly developing embryo.
IN VITRO FERTILISATION METHODS
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